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Experimental design

Practice of microiontophoresis in an Automated System

The widespread usage of high-performance microcomputers and the advent of relatively low-cost data acquisition and instrument control plug-in boards allow a high degree of automation in biomedical experiments including iontophoretic application of neuroactive substances from multibarrel pipettes. In complex microiontophoresis experiment, channels of the iontophoresis pump are gated by the computer according to a preprogrammed time pattern and the firing rate of the neuron under investigation is simultaneously recorded. A possible experimental design of such kind is schematically illustrated on this page along with references dealing with LabVIEW application in Neuroscience or Physiology. For more information e-mail to info@kationscientific.com.


 

 

Driver software:

Our Ratemeter & Timer software program is created in a LabVIEW environment. It controls automated multichannel iontophoresis experiments in conjunction with PCI-1200 plug-in boards made by National Instruments.

Extracellular recording:

We have developed a simple yet professional quality amplifier for neuronal spike recording. The ExAmp-20KB excels in low-noise extracellular recordings taken through carbon fiber or tungsten electrodes.

Iontophoresis pumps:

We offer our Union-40 nanoampere and BAB-501 microampere iontophoresis pumps designed to deliver currents in the respective ranges of 0-200 nA and 1-20 µA.

Microelectrodes:

Carbon fiber microelectrodes permit low-noise extracellular recordings. They can also be used for voltammetric analysis of transmitters in vivo. We offer three types of carbon fiber electrodes for sale. For more, see our Micropipettes and Order and pricelist pages.


 

 

 

 

 

 

 

References: LabVIEW applications in Neuroscience and Physiology
Angrilli, A. PSAAL: A LabVIEW 3 program for data acquisition and analysis in psychophysiological experiments. Behavior Research Methods Instruments & Computers, v.27, n.3, (1995): 367-374.
Budai, D. A computer-controlled system for post-stimulus time histogram and wind-up studies. Journal of Neuroscience Methods, v.51, n.2, (1994): 205-211.
Budai, D; Kehl, L J; Poliac, G I; Wilcox, G L. An Iconographic Program for Computer-Controlled Whole-Cell Voltage Clamp Experiments. Journal of Neuroscience Methods, v.48, n.1-2, (1993): 65-74.
Davis, G; Johns, E J; Ross, H F. Data Acquisition Using the Apple Macintosh and Labview Software Recording Physiological Responses in the Rat During Renal Functional Studies. Journal of Physiology (Cambridge), v.435, (1991):.
Ellis, W S; Jones, R T. Using LabView to facilitate calibration and verification for respiratory impedance plethysmography. Computer Methods and Programs in Biomedicine, v.36, n.4, (1991): 169-176.
Gottschaldt, K-M; Hicks, T P; Vahle-Hinz, C. A combined recording and microiontophoresis technique for input-output analysis of single neurons in the mammalian CNS. Journal of Neuroscience Methods, v.23, (1988): 233-239.
Gulotta, M. Teaching computer interfacing with virtual instruments in an object-oriented language. Biophysical Journal, v.69, n.5, (1995): 2168-2173.
Kaelin-Lang, A; Niemeyer, G. A PC-program for the analysis of electrophysiological signals recorded from the mammalian eye. Klinische Monatsblaetter fuer Augenheilkunde, v.206, n.5, (1995): 394-396. Language: German.
Kling-Petersen, T; Svensson, K. A Simple Computer-Based Method for Performing and Analyzing Intracranial Self-Stimulation Experiments in Rats. Journal of Neuroscience Methods, v.47, n.3, (1993): 215-225.
Laouris, Y; Reinking, R M; Stuart, D G. Computer-aided extraction of the features of the EMG of single motor units. Brain Research Bulletin, v.26, n.6, (1991): 997-1002.
Nordstrom, M A; Mapletoft, E A; Miles, T S. Spike-train acquisition, analysis and real-time experimental control using a graphical programming language (LabView). Journal of Neuroscience Methods, v.62, n.1-2, (1995): 93-102.
O'Grady, K F; Doyle, D J. Characterization and evaluation of diastolic heart function using the LabVIEW signal processing environment. Journal of Clinical Engineering, v.20, n.4, (1995): 311-331.
Poindessault, J-P; Beauquin, C; Gaillard, F. Stimulation, data acquisition, spikes detection and time-rate analysis with a graphical programming system: An application to vision studies. Journal of Neuroscience Methods, v.59, n.2, (1995): 225-235.
Swanson, H D. A Comparison for Teaching Purposes of Three Data-Acquisition Systems for the Macintosh. American Journal of Physiology, v.258, n.6 PART 3, (1990): S17-S23.
Wong, L; Sissons, C H; Cutress, T W. Control of a multiple dental plague culture system and long-term, continuous, plague pH measurement using LabVIEW. Binary Computing in Microbiology, v.6, n.5, (1994): 173-180.
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